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Exploring the incorporation of mulberry (Morus Alba L.) into freeze-dried yogurt for enhanced nutritional value and quality
Corresponding Author(s) : Thanh Viet Nguyen
Carpathian Journal of Food Science and Technology,
Vol. 15 No. 3 (2023): Carpathian Journal of Food Science and Technology
Abstract
Yogurt, popular worldwide, is nutrient-rich with calcium, potassium, protein, B vitamins, and beneficial microorganisms. Mulberries possess medicinal qualities, notably anthocyanins, which have antioxidant, antidiabetic, and anti-bacterial effects. Though fruit-flavored yogurt is popular, producing it is challenging because of its limited shelf life. However, freezedrying technology can address this, allowing prolonged storage without compromising its quality. This study evaluated incorporating mulberry into yogurt to enhance its nutritional value and quality while seeking an optimal production process for freeze-dried mulberry yogurt. Mulberry syrup was added in various ratios, demonstrating statistically significant effects on the yogurt's physicochemical properties. Particularly, a 9:1 mulberry syrup to yogurt ratio exhibited a total polyphenol content of 170.84 mg GAE/100 g, marking a 94% increase compared to the yogurt without mulberry syrup. This substantial enhancement signifies an improvement in yogurt efficiency when mulberry is integrated. Similarly, DPPH and ABTS free radical scavenging activities for the 9:1 ratio were observed at 12.22 mgAAE/100g and 22.55 mgAAE/100g, which is an enhancement of 1.89% and 15.99%, respectively, compared to the reference yogurt sample (10:0 ratio). The quality changes in the freeze-dried mulberry yogurt were monitored over a 28-day storage period. The results revealed relatively stable physicochemical properties, microbiological density, and biological activity throughout the storage, highlighting the potential of freeze-drying technology in creating innovative and nutritious yogurt products.
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- Arfaoui, L. (2020). Total polyphenol content and radical scavenging activity of functional yogurt enriched with dates. Czech Journal of Food Sciences, 38(5), 287-292.
- Barbosa, J., Borges, S., Amorim, M., Pereira, M. J., Oliveira, A., Pintado, M. E., Teixeira, P. (2015). Comparison of spray drying, freeze drying and convective hot air drying for the production of a probiotic orange powder. Journal of Functional Foods, 17, 340–351.
- Bosnea, L. A., Kopsahelis, N., Kokkali, V., Terpou, A., Kanellaki, M. (2017). Production of a novel probiotic yogurt by incorporation of L. casei enriched fresh apple pieces, dried raisins and wheat grains. Food and Bioproducts Processing, 102, 62–71.
- Butt, M. S., Nazir, A., Sultan, M. T., Schroën, K. (2008). Morus alba L. nature’s functional tonic. Trends in Food Science & Technology, 19(10), 505–512.
- Calín-Sánchez, Á., Martínez-Nicolás, J. J., Munera-Picazo, S., Carbonell-Barrachina, Á. A., Legua, P., Hernández, F. (2013). Bioactive compounds and sensory quality of black and white mulberries grown in Spain. Plant Foods for Human Nutrition, 68, 370–377.
- Chen, C., Mokhtar, R. A. M., Sani, M. S. A., Noor, N. Q. I. M. (2022). The Effect of Maturity and Extraction Solvents on Bioactive Compounds and Antioxidant Activity of Mulberry (Morus alba) Fruits and Leaves. Molecules, 27(8), 2406.
- Dai, J., Yu, G., Ou, W., Ai, Q., Zhang, W., Mai, K., Zhang, Y. (2021). Dietary supplementation of stachyose and Lactobacillus casei improves the immunity and intestinal health of turbot (Scophthalmus maximus. L). Aquaculture Nutrition, 27, 48–60.
- Deshwal, G. K., Tiwari, S., Kumar, A., Raman, R. K., Kadyan, S. (2021). Review on factors affecting and control of post-acidification in yoghurt and related products. Trends in Food Science & Technology, 109, 499–512.
- Desobry-Banon, S., Vetier, N., Hardy, J. (1999). Health benefits of yogurt consumption. A review. International Journal of Food Properties, 2(1), 1–12.
- Bae, S. H., Suh, H. J. (2007). Antioxidant activities of five different mulberry cultivars in Korea. LWT-Food Science and Technology, 40(6), 955–962.
- Emteborg, H., Charoud-Got, J., Seghers, J. (2022). Infrared Thermography for Monitoring of Freeze Drying Processes— Part 2: Monitoring of Temperature on the Surface and Vertically in Cuvettes during Freeze Drying of a Pharmaceutical Formulation. Pharmaceutics, 14(5), 1007.
- Ermis, E. (2022). A review of drying methods for improving the quality of probiotic powders and characterization. Drying Technology, 40(11), 2199–2216.
- Fellows, P. (2017). Freeze drying and freeze concentration. Food Processing Technology, 4th Edn. Woodhead Publications Ltd., England, 441–451.
- Gilliland, S. E. (1989). Acidophilus milk products: A review of potential benefits to consumers. Journal of Dairy Science, 72(10), 2483–2494.
- Golmakani, M. T., Eskandari, M. H., Kooshesh, S., Pishan, M. (2021). Investigation of the effects of pomegranate juice addition on physicochemical, microbiological, and functional properties of set and stirred yogurts. Food Science & Nutrition, 9(12), 6662–6671.
- Grace, M. H., Ribnicky, D. M., Kuhn, P., Poulev, A., Logendra, S., Yousef, G. G., Raskin, I., Lila, M. A. (2009). Hypoglycemic activity of a novel anthocyanin-rich formulation from lowbush blueberry, Vaccinium angustifolium Aiton. Phytomedicine, 16(5), 406–415.
- Ha, H. P., Trang, V. T., Nghia, N. C., Son, V. H. (2021). Development of Freeze-Dried Red Dragon Fruit Yoghurt Containing Probiotics. Red, 31(4), 014–018.
- Jovanović, M., Petrović, M., Miočinović, J., Zlatanović, S., Laličić Petronijević, J., Mitić-Ćulafić, D., Gorjanović, S. (2020). Bioactivity and sensory properties of probiotic yogurt fortified with apple pomace flour. Foods, 9(6), 763.
- Lee, J., Durst, R., and Wrolstad, R. (2005). AOAC official method 2005.02: Total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method. Official Methods of Analysis of AOAC International, 2.
- Lim, Y. Y., Lim, T. T., Tee, J. J. (2007). Antioxidant properties of several tropical fruits: A comparative study. Food Chemistry, 103(3), 1003–1008.
- Lin, J. Y., Tang, C. Y. (2007). Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chemistry, 101(1), 140–147.
- Liu, B., Zhou, X. (2015). Freeze-drying of proteins. Cryopreservation and FreezeDrying Protocols, 459–476.
- Liu, L. K., Chou, F. P., Chen, Y. C., Chyau, C. C., Ho, H.-H., Wang, C.-J. (2009). Effects of mulberry (Morus alba L.) extracts on lipid homeostasis in vitro and in vivo. Journal of Agricultural and Food Chemistry, 57(16), 7605–7611.
- Lutchmedial, M., Ramlal, R., Badrie, N., Chang-Yen, I. (2004). Nutritional and sensory quality of stirred soursop (Annona muricata L.) yoghurt. International Journal of Food Sciences and Nutrition, 55(5), 407–414.
- McDonough, F. E., Hitchins, A. D., Wong, N. P. (1982). Effects of yogurt and freeze-dried yogurt on growth stimulation of rats. Journal of Food Science, 47(5), 1463–1465.
- Miller, G. D., Jarvis, J. K., and McBean, L. D. (2006). Handbook of dairy foods and nutrition. CRC press.
- Mostafa, S., Wang, Y., Zeng, W., Jin, B. (2022). Floral scents and fruit aromas: Functions, compositions, biosynthesis, and regulation. Frontiers in Plant Science, 13.
- Okatan, V., Polat, M., Aşkin, M. A. (2016). Some physico-chemical characteristics of black mulberry (Morus nigra L.) in Bitlis. Scientific Papers-Series B, Horticulture, 60, 27–30.
- Peryam, D. R., Pilgrim, F. J. (1957). Hedonic scale method of measuring food preferences. Food Technology.
- Phuong, N. N. M., Le, T. T., Dang, M. Q., Van Camp, J., Raes, K. (2020). Selection of extraction conditions of phenolic compounds from rambutan (Nephelium lappaceum L.) peel. Food and Bioproducts Processing, 122, 222–229.
- Punthi, F., Jomduang, S. (2021). Effect of processing steps on bioactive compounds and antioxidant activities of high anthocyanin mulberry fruit powder. CMUJ. Nat. Sci, 20(2), e2021043.
- Rizzoli, R. (2014). Dairy products, yogurts, and bone health. The American Journal of Clinical Nutrition, 99(5), 1256S-1262S.
- Saensouk, S., Senavongse, R., Papayrata, C., Chumroenphat, T. (2022). Evaluation of Color, Phytochemical Compounds and Antioxidant Activities of Mulberry Fruit (Morus alba L.) during Ripening. Horticulturae, 8(12), 1146.
- Sangteerakij, D., Rodyu, S., Junden, S. (2023). Nutrition composition and anthocyanin content of mulberry fruits (Morus alba L.) by different ripening stage grown in the south of Thailand, applicants in sorbet and sherbet ice-cream and analysis of physical, chemical and sensory evaluation. Emirates Journal of Food and Agriculture.
- Suh, H. J., Kim, J. M., Lee, H., Lee, S. W., Choi, Y. M. (2004). Thermal kinetics on antiradical capacity of mulberry fruit extract. European Food Research and Technology, 219, 80–83.
- Sung, J. M., Kim, Y. B., Kum, J. S., Choi, Y. S., Seo, D. H., Choi, H. W., Park, J. D. (2015). Effects of freeze-dried mulberry on antioxidant activities and fermented characteristics of yogurt during refrigerated storage. Korean Journal for Food Science of Animal Resources, 35(6), 807.
- Sun-Waterhouse, D., Zhou, J., Wadhwa, S. S. (2012). Effects of adding apple polyphenols before and after fermentation on the properties of drinking yoghurt. Food and Bioprocess Technology, 5, 2674–2686.
- Tapia, M. S., Alzamora, S. M., Chirife, J. (2020). Effects of water activity (aw) on microbial stability as a hurdle in food preservation. Water Activity in Foods: Fundamentals and Applications, 323–355.
- Tuyen, C. K., Nguyen, M. H., Roach, P. D., Stathopoulos, C. E. (2015). A storage study of encapsulated gac (Momordica cochinchinensis) oil powder and its fortification into foods. Food and Bioproducts Processing, 96, 113–125.
- Valentina, V., Pratiwi, R. A., Hsiao, P. Y., Tseng, H. T., Hsieh, J. F., Chen, C. C. (2016). Sensorial characterization of foods before and after freeze-drying. Sensorial Characterization of Foods Before and After Freeze-Drying, 1(6), 1–5.
- Wang, C., Cheng, W., Bai, S., Ye, L. I., Du, J., Zhong, M., Liu, J., Zhao, R., Shen, B. (2019). White mulberry fruit polysaccharides enhance endothelial nitric oxide production to relax arteries in vitro and reduce blood pressure in vivo. Biomedicine & Pharmacotherapy, 116, 109022.
- Wang, H., Cao, G., Prior, R. L. (1997). Oxygen radical absorbing capacity of anthocyanins. Journal of Agricultural and Food Chemistry, 45(2), 304–309.
- Yawadio, R., Morita, N. (2007). Color enhancing effect of carboxylic acids on anthocyanins. Food Chemistry, 105(1), 421–427.
- Yuan, Q., Zhao, L. (2017). The Mulberry (Morus alba L.) Fruit A Review of Characteristic Components and Health Benefits. Journal of Agricultural and Food Chemistry, 65(48), 10383–10394.
References
Arfaoui, L. (2020). Total polyphenol content and radical scavenging activity of functional yogurt enriched with dates. Czech Journal of Food Sciences, 38(5), 287-292.
Barbosa, J., Borges, S., Amorim, M., Pereira, M. J., Oliveira, A., Pintado, M. E., Teixeira, P. (2015). Comparison of spray drying, freeze drying and convective hot air drying for the production of a probiotic orange powder. Journal of Functional Foods, 17, 340–351.
Bosnea, L. A., Kopsahelis, N., Kokkali, V., Terpou, A., Kanellaki, M. (2017). Production of a novel probiotic yogurt by incorporation of L. casei enriched fresh apple pieces, dried raisins and wheat grains. Food and Bioproducts Processing, 102, 62–71.
Butt, M. S., Nazir, A., Sultan, M. T., Schroën, K. (2008). Morus alba L. nature’s functional tonic. Trends in Food Science & Technology, 19(10), 505–512.
Calín-Sánchez, Á., Martínez-Nicolás, J. J., Munera-Picazo, S., Carbonell-Barrachina, Á. A., Legua, P., Hernández, F. (2013). Bioactive compounds and sensory quality of black and white mulberries grown in Spain. Plant Foods for Human Nutrition, 68, 370–377.
Chen, C., Mokhtar, R. A. M., Sani, M. S. A., Noor, N. Q. I. M. (2022). The Effect of Maturity and Extraction Solvents on Bioactive Compounds and Antioxidant Activity of Mulberry (Morus alba) Fruits and Leaves. Molecules, 27(8), 2406.
Dai, J., Yu, G., Ou, W., Ai, Q., Zhang, W., Mai, K., Zhang, Y. (2021). Dietary supplementation of stachyose and Lactobacillus casei improves the immunity and intestinal health of turbot (Scophthalmus maximus. L). Aquaculture Nutrition, 27, 48–60.
Deshwal, G. K., Tiwari, S., Kumar, A., Raman, R. K., Kadyan, S. (2021). Review on factors affecting and control of post-acidification in yoghurt and related products. Trends in Food Science & Technology, 109, 499–512.
Desobry-Banon, S., Vetier, N., Hardy, J. (1999). Health benefits of yogurt consumption. A review. International Journal of Food Properties, 2(1), 1–12.
Bae, S. H., Suh, H. J. (2007). Antioxidant activities of five different mulberry cultivars in Korea. LWT-Food Science and Technology, 40(6), 955–962.
Emteborg, H., Charoud-Got, J., Seghers, J. (2022). Infrared Thermography for Monitoring of Freeze Drying Processes— Part 2: Monitoring of Temperature on the Surface and Vertically in Cuvettes during Freeze Drying of a Pharmaceutical Formulation. Pharmaceutics, 14(5), 1007.
Ermis, E. (2022). A review of drying methods for improving the quality of probiotic powders and characterization. Drying Technology, 40(11), 2199–2216.
Fellows, P. (2017). Freeze drying and freeze concentration. Food Processing Technology, 4th Edn. Woodhead Publications Ltd., England, 441–451.
Gilliland, S. E. (1989). Acidophilus milk products: A review of potential benefits to consumers. Journal of Dairy Science, 72(10), 2483–2494.
Golmakani, M. T., Eskandari, M. H., Kooshesh, S., Pishan, M. (2021). Investigation of the effects of pomegranate juice addition on physicochemical, microbiological, and functional properties of set and stirred yogurts. Food Science & Nutrition, 9(12), 6662–6671.
Grace, M. H., Ribnicky, D. M., Kuhn, P., Poulev, A., Logendra, S., Yousef, G. G., Raskin, I., Lila, M. A. (2009). Hypoglycemic activity of a novel anthocyanin-rich formulation from lowbush blueberry, Vaccinium angustifolium Aiton. Phytomedicine, 16(5), 406–415.
Ha, H. P., Trang, V. T., Nghia, N. C., Son, V. H. (2021). Development of Freeze-Dried Red Dragon Fruit Yoghurt Containing Probiotics. Red, 31(4), 014–018.
Jovanović, M., Petrović, M., Miočinović, J., Zlatanović, S., Laličić Petronijević, J., Mitić-Ćulafić, D., Gorjanović, S. (2020). Bioactivity and sensory properties of probiotic yogurt fortified with apple pomace flour. Foods, 9(6), 763.
Lee, J., Durst, R., and Wrolstad, R. (2005). AOAC official method 2005.02: Total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method. Official Methods of Analysis of AOAC International, 2.
Lim, Y. Y., Lim, T. T., Tee, J. J. (2007). Antioxidant properties of several tropical fruits: A comparative study. Food Chemistry, 103(3), 1003–1008.
Lin, J. Y., Tang, C. Y. (2007). Determination of total phenolic and flavonoid contents in selected fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chemistry, 101(1), 140–147.
Liu, B., Zhou, X. (2015). Freeze-drying of proteins. Cryopreservation and FreezeDrying Protocols, 459–476.
Liu, L. K., Chou, F. P., Chen, Y. C., Chyau, C. C., Ho, H.-H., Wang, C.-J. (2009). Effects of mulberry (Morus alba L.) extracts on lipid homeostasis in vitro and in vivo. Journal of Agricultural and Food Chemistry, 57(16), 7605–7611.
Lutchmedial, M., Ramlal, R., Badrie, N., Chang-Yen, I. (2004). Nutritional and sensory quality of stirred soursop (Annona muricata L.) yoghurt. International Journal of Food Sciences and Nutrition, 55(5), 407–414.
McDonough, F. E., Hitchins, A. D., Wong, N. P. (1982). Effects of yogurt and freeze-dried yogurt on growth stimulation of rats. Journal of Food Science, 47(5), 1463–1465.
Miller, G. D., Jarvis, J. K., and McBean, L. D. (2006). Handbook of dairy foods and nutrition. CRC press.
Mostafa, S., Wang, Y., Zeng, W., Jin, B. (2022). Floral scents and fruit aromas: Functions, compositions, biosynthesis, and regulation. Frontiers in Plant Science, 13.
Okatan, V., Polat, M., Aşkin, M. A. (2016). Some physico-chemical characteristics of black mulberry (Morus nigra L.) in Bitlis. Scientific Papers-Series B, Horticulture, 60, 27–30.
Peryam, D. R., Pilgrim, F. J. (1957). Hedonic scale method of measuring food preferences. Food Technology.
Phuong, N. N. M., Le, T. T., Dang, M. Q., Van Camp, J., Raes, K. (2020). Selection of extraction conditions of phenolic compounds from rambutan (Nephelium lappaceum L.) peel. Food and Bioproducts Processing, 122, 222–229.
Punthi, F., Jomduang, S. (2021). Effect of processing steps on bioactive compounds and antioxidant activities of high anthocyanin mulberry fruit powder. CMUJ. Nat. Sci, 20(2), e2021043.
Rizzoli, R. (2014). Dairy products, yogurts, and bone health. The American Journal of Clinical Nutrition, 99(5), 1256S-1262S.
Saensouk, S., Senavongse, R., Papayrata, C., Chumroenphat, T. (2022). Evaluation of Color, Phytochemical Compounds and Antioxidant Activities of Mulberry Fruit (Morus alba L.) during Ripening. Horticulturae, 8(12), 1146.
Sangteerakij, D., Rodyu, S., Junden, S. (2023). Nutrition composition and anthocyanin content of mulberry fruits (Morus alba L.) by different ripening stage grown in the south of Thailand, applicants in sorbet and sherbet ice-cream and analysis of physical, chemical and sensory evaluation. Emirates Journal of Food and Agriculture.
Suh, H. J., Kim, J. M., Lee, H., Lee, S. W., Choi, Y. M. (2004). Thermal kinetics on antiradical capacity of mulberry fruit extract. European Food Research and Technology, 219, 80–83.
Sung, J. M., Kim, Y. B., Kum, J. S., Choi, Y. S., Seo, D. H., Choi, H. W., Park, J. D. (2015). Effects of freeze-dried mulberry on antioxidant activities and fermented characteristics of yogurt during refrigerated storage. Korean Journal for Food Science of Animal Resources, 35(6), 807.
Sun-Waterhouse, D., Zhou, J., Wadhwa, S. S. (2012). Effects of adding apple polyphenols before and after fermentation on the properties of drinking yoghurt. Food and Bioprocess Technology, 5, 2674–2686.
Tapia, M. S., Alzamora, S. M., Chirife, J. (2020). Effects of water activity (aw) on microbial stability as a hurdle in food preservation. Water Activity in Foods: Fundamentals and Applications, 323–355.
Tuyen, C. K., Nguyen, M. H., Roach, P. D., Stathopoulos, C. E. (2015). A storage study of encapsulated gac (Momordica cochinchinensis) oil powder and its fortification into foods. Food and Bioproducts Processing, 96, 113–125.
Valentina, V., Pratiwi, R. A., Hsiao, P. Y., Tseng, H. T., Hsieh, J. F., Chen, C. C. (2016). Sensorial characterization of foods before and after freeze-drying. Sensorial Characterization of Foods Before and After Freeze-Drying, 1(6), 1–5.
Wang, C., Cheng, W., Bai, S., Ye, L. I., Du, J., Zhong, M., Liu, J., Zhao, R., Shen, B. (2019). White mulberry fruit polysaccharides enhance endothelial nitric oxide production to relax arteries in vitro and reduce blood pressure in vivo. Biomedicine & Pharmacotherapy, 116, 109022.
Wang, H., Cao, G., Prior, R. L. (1997). Oxygen radical absorbing capacity of anthocyanins. Journal of Agricultural and Food Chemistry, 45(2), 304–309.
Yawadio, R., Morita, N. (2007). Color enhancing effect of carboxylic acids on anthocyanins. Food Chemistry, 105(1), 421–427.
Yuan, Q., Zhao, L. (2017). The Mulberry (Morus alba L.) Fruit A Review of Characteristic Components and Health Benefits. Journal of Agricultural and Food Chemistry, 65(48), 10383–10394.